-
Hi,
I hope you are well.
I am kindly writing to learn whether it is possible to use Sniffles2 to investigate SVs in direct RNA sequencing data. I have seen in previous issues that there is a relat…
-
Hello,
I am attempting to use the PLIER package for pseudobulk analysis of single-cell RNA sequencing data. I have generated pseudobulk data using the PseudobulkExpression function from Seurat and …
-
I have this samplesheet here that should be fine and passing validation, but I cannot get this to work.
```bash
[Header]
FileFormatVersion,2
RunName,name123456
InstrumentPlatform,NovaSeq
oper…
-
**DO NOT INCLUDE REQUESTS IN THE FIRST COMMENT.**
**PLEASE POST THIS TEMPLATE UNCHANGED THEN FOLLOW ITS INSTRUCTIONS IN A NEW
COMMENT**
# General Notes
This repository serves as a mostly automated p…
-
Hey there
does this tool support Direct RNa seq data? i ran Direct RNA sequencing using SQK-RNA004 library kit.
thank you
-
**Work Flow:** RNA v4
**Protocol:** Normalization of RNA samples for sequencing
**Step:** Normalization of RNA samples for sequencing
## **Description:**
_Describe the reason for the verification a…
-
## patch-seq assay
Label: patch-seq assay
Alternative term(s): patch clamp and RNA sequencing assay
Definition: A single-cell RNA sequencing assay that takes a patch clamp recording from a cell a…
-
I really like your preprint and I would love to use it for direct RNA sequencing.
Do you happen to work on adjusting the UNCALLED for direct RNA sequencing? Given that an accurate RNA k-mer model is …
-
Hello,
Question about 'RNA sequencing assay' (OBI:0001177) and 'RNA-seq assay' (OBI:0001271). In OBI, 'RNA-seq assay' is a subclass of 'RNA sequencing assay'. Are there examples of assays that fal…
-
Before this change https://github.com/samtools/htslib/pull/1854 U was changed to N when read by samtools
Now it will be changed to T
However, I think it would be "better" if we could preserve U …