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Best SRA team,
We were granted an access to phs001680.v1.p1 data from dbGap. I downloaded all the scRNAseq (Smart-seq2) files with prefetch command and I have been trying to convert the SRA files t…
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Dear professor
We're testing CNV for glioblastoma
When we use 10x glioblastoma data, the CNV heat map is consistent with disease characteristics（chr7 amplification, chr10 deletion）
But when we use …
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pbmcMultiome as described in this vignettes :https://satijalab.org/seurat/articles/atacseq_integration_vignette.html for some reason is not available to download. How to fix the issue
```
Instal…
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https://mp.weixin.qq.com/s/NvB9Key3gbWGewlFUIIVMg
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Datasets are expected to provide raw count data; however, `load_tabula_muris_senis` returns normalized counts. This is causing #612 to fail and likely also #611, as well as probably giving erroneous r…
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### Description of feature
I want to adapt the pipeline for SMART-Seq2 as well. There exist a SMART-seq2 nf-core pipeline (https://github.com/nf-core/smartseq2), but it is outdated and the last commi…
heylf updated
2 years ago
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Hi kallisto contributors,
kallisto is an amazing tool for alignment of RNA-seq/scRNA-seq, i'm using kb for smart-seq2 data and got an Error when running kb count with
`kb count -i macaca_fasci…
x1han updated
2 years ago
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您好，我在用PISA分析DNBelab C4平台的单细胞RNAseq数据。
我看manual中，`Quickly start`部分后面还有对细胞和分子进行去重复的操作，请问这是必需的吗？理论上PCR重复应该有相同的UMI，它们应该早在`PISA corr`这一步就被去除了。
`# Deduplicate BAM file for each cell`
`$ PISA rmdup -tags …
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* identify dataset
* add instructions to generate the rawdata (don't check-in the rawdata to the repo!)
* add the necessary config files to run the pipeline with the dataset (targets, contrasts, etc…
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**Describe the issue**
Hi!
I met an error when I run `kb count` to generate a gene count matrix, the output `Error: Number of files (2) does not match number of input files required by technology BU…