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The dystrophin like protein (dlp) is being employed as a hard test case while building transcriptomes for several different sea urchin species. The full length dlp mRNA is more than 13kbp and the pr…
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Hi,
I assembled a plant transcriptome from ~350M 50x50 PE reads using Trinity 2.6.6 with the following (relevant) parameters:
````
--no_normalize_reads --no_path_merging --min_kmer_cov 5
````
…
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Hi Brian,
As I mentioned during our meeting ~2 weeks ago, we have an issue that Trinity does not assemble full-length genes that should be present in the transcriptome, because we already cloned th…
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Hi,
I am working on the single cell RNA seq data from the 10x genomics pipeline. The fastq file generate by this pipeline put all the cells from one sample together. Is there any way I can demultiple…
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hi ,
I am delighted to use minimap for preprocessing when using large data. However, the number of transcripts in the different partition is unbalanced, with one of them reaching tens of thousand…
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hi, Dear
when I test reconstruct_contig.py，there is a problem as follows：
reconstruct_contig.py -h
Traceback (most recent call last):
File "/home/software/Cogent/software/Anaconda2/en…
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I am running `tracer test` and not replicating the expected summary that is included with the release tracer-0.6.0. I am only detecting a single clonotype, not two clonotypes with 1 and 2 clones each.…
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reported by @TKlingstrom
Server error while importing following workflow to test.galaxyproject.org
corresponding sentry entry: https://sentry.galaxyproject.org/sentry/test/issues/290644/
https…
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I am trying to perform stringtie --merge:
The contents of the folder where I want to merge are :
ACI-SegHsd-brain_ST.gtf F344-Ncl-brain_ST.gtf SR-JrHsd1-brain_ST.gtf
Cop-CrCrl_brain_ST.gt…
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## **For the latest pre-release version, 2.6.2, In the top source directory after decompressing I issued:**
make
.
.
.
make[1]: Leaving directory `Programs/trinityrnaseq-2.6.2-prerelease/trinit…