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I read in Lior's preprint X thread that splitcode would work well with long-reads, do you have any example of how to implement it for this case?
I would like to test it to demultiplex single-cell lo…
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Dear BLAZE developers,
I was wondering if the BLAZE pipeline can be used with a different single-cell protocol than 10X.
If our single-cell protocol has a different Adapter sequence, and we have …
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Dear Velocyto Team,
Our lab used a modified Smart-seq2 protocol to allow for multiplexed single-cell RNA-seq. Primers of RT reaction were designed with cell-specific barcodes and unique molecular ide…
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Hi,
I'm currently working with a dataset from Yuan et al. 2018 (GSE103224). They did something similar to Drop-seq, but using Illumina Nextera-XT. They state in their paper that read1 contains cell…
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比如这个数据已经经过了如下的处理，可以用您们的包直接读入吗？
Single-cell suspensions were converted to barcoded scRNA-seq libraries by using the Chromium Single Cell 3’ Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics…
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Hi AMULET team,
I have a 10X multiome kit dataset and trying to run AMULET on the ATAC data.
The fragments input file is the same, but the singlecell.csv is no longer available as CellRanger outpu…
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**User story**
As a user of the scRNA Core Cell Extraction pipeline, if I have made multiple sets of child tubes from the LRC PBMC Bank plate, I would like to be able to download the driver file for …
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Thanks for the great tool!
I recently introduced `kana` in Bio"Pack"athon, which is my hackathon community in Japan, and there were some positive reactions.
cf. Sorry for that it is in Japanese...…
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Hi Tim,
Thanks for developing this tool! It's very handy. Just one question: If I want to merge the bam files from multiple samples after running ```sinto filterbarcodes```, how can I avoid the issue…
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I'm using STARsolo on a 10X v2 scRNA-seq dataset where the barcode read contained the CB + UMI sequence is not 26 base pairs but 150 base pairs in length. In order to run STAR solo, I set the --soloBa…