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@golu099 brought up [GAPPadder](https://github.com/simoncchu/GAPPadder) as a tool to potentially replace [Gap2Seq](https://github.com/rikuu/Gap2Seq) for filling gaps in seq coverage between scaffolded…
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Hello,
I would like to use your software on sequencing data aligned to the newest R. norvegicus genome (RGSC 6.0/rn6). Could you please add it to your annotation database?
Cheers,
alussier17
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Hi,
I am trying to assemble the genome of a worm, and I was hoping that minipolish would detect the mitochondrial genome and output it in the gfa file.
I have sequencing one of my worm species a l…
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The option to select Illumina, Nanopore, etc is included on the interface for the assembly service, but is not provided in the CGA service. This came to my intention when a user noted that she was as…
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This command consumed about 60G RAM. But it phased only 10Mb area. I still left about 2500Mb area to phase. how can I reduce the RAM when phasing a large cohort (~1200) of whole-genome sequencing data…
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Dear Unicycler team,
I chose Unicycler to assemble _de novo_ bacterial genomes with ONT sequences, because I tried with several ones and Unicycler gived me better results.
However I start to obser…
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Hi, do you think that this method could be applied to high coverage >80x whole genome sequencing? Also will this only identify fusions from simple rearrangements or also from multi-breakpoint events?
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Hi. Let me describe my data to see if I can use XYalign to find Y-contigs
My data is whole genome re-sequencing data with coverage of ~ 15X, including male and female fish (around 20 each sex)
I h…
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Is it possible to use the tool for evaluating correction of the cDNA reads obtained for transcriptome sequencing? Would it be enough to substitute reference genome with the reference transcriptome?
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So far, usually, users upload mostly vcf files that do not have reference alleles. At the same time, big part of the annotation information (especially in our annotator) is about the reference alleles…