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Dear Dengfeng,
Thanks for your tool.
I tried to use purge_dups to clean our assembly. I have some questions:
1. we have Pacbio sequencing data and Illumina sequencing data, I want to use all of …
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Hi,
I have a PacBio WGS BAM (whose reference genome is hg19) from a tumor, and I would like to use "CoRAL"
When I visited this site, I found there is a file like "https://github.com/AmpliconSuite…
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Hi,
I installed pacbio_qc with pip install and ran below two commands
` sequana_pacbio_qc --input-directory /data/shared/clinical/LongRead/Data/`
input dir where i have one of BAM file GIAB Tri…
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Hello,
I am trying to use bcftools 1.17 to call variants in bacterial genomes with PacBio CCS data.
I attempted to use the mapped CSS reads, but kept running into segmentation fault errors and g…
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Hello,
Just a quick query on adapter dectection, there is smrtbell adapter which keep poping up in our dataset, and looking blasting it at ncbi, not only ours.
I would be nice if it was possibl…
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Hi, I'm just wondering if VarTrix can be used for variant detection in ONT long-read data or is it just exclusively for short-read single cell sequencing data? Has anyone tried this and succeeded?
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Hello,
I am very interested in using your approach for binning HiFi reads. I plan to test it on some internal datasets, but wanted to make you aware of a new dataset I have made available on NCBI. Th…
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Hello, thanks for the great resource!
Just a heads up that some files under the `PacBio/` folder are not publicly accessible.
For example:
```bash
# This works
➜ Desktop aws s3 cp s3://dna…
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Hi @pb-jchin @pb-cdunn ,
Please help me with this issue, I was running falcon_unzip 0.4.0, and was successful using Ecoli example data after falcon running. However, it was failed when I ran my own…
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Hi,
I am trying to load a gtf file but run into an error I could not solve using the importGTF function:
>
> Step 1 of 10: Checking data...
> Step 2 of 10: Obtaining annotation...
> imp…