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How does DIA-NN recognize decoys in a spectral library? Is it based on the column "Decoy" as shown below in the library?
| Decoy |
|---|
| No |
| No |
| Yes |
Bo
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Hello! I am running DiaNN 1.8 on Windows and I noticed something odd, and I was wondering if there was an explanation for it. I basically have two runs.
All in One: Running all the samples at once …
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Hi,
Thank you so much for creating DIA-NN. I am new to this tool. I was trying to generate a predicted spectral library from a FASTA file. According to the GUI of DIA-NN, I should get a spectral li…
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Hello, I encountered an issue as described in the title.
Building Spectral Library using DIA-NN v1.9.1
I created a spectral library using DIA-NN 1.9.1 with the following parameters. The output f…
Mnago updated
3 months ago
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The class `de.ipbhalle.metfraglib.scoreinitialisation.OfflineMetFusionSpectralSimilarityScoreInitialiser`
at https://github.com/ipb-halle/MetFragRelaunched/blob/c57f9d2b406350b2357ce9f7ce42a286cefcca…
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Dear Vadim,
I am interested in performing quantitative phosphoproteomic using library-free mode. The library-free mode is fast when doing unmodified peptide search but I am concerned about the sear…
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https://speclib.jpl.nasa.gov/
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Hello Vadim,
I tried to load a spectral library from this repository: https://github.com/ZiluYe/Glyco-DIA
These are generated spectral libraries with glyco modifications from Spectronaut.
When…
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Hello,
I am trying to create an in-silico predicted spectral library in DIA-NN and I am interested in detecting variable carbamylation of lysine (K) and arginine (R), as well as the carbamylation a…
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Would it be possible to add a feature that allows users to identify the spectral library uploaded that was created in-house (i.e. the one with the highest accuracy) such that matches made using that l…