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Dear GitHub Community, can anyone help me with the following problem, please?
We treated two different cell lines with our substance of interest (in triplicates) and had RNA sequencing analysis don…
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Dear m6anet-team,
I have a question regarding m6anet, which I try to use in Galaxy to reproduce some RNA-sequencing study in order to get familiar with this topic.
In your manual you write for dat…
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Dear pychopper developers,
I have been comparing the difference in trimming between `dorado trim` tool and pychopper with `-m edlib` option activated and only using the full-length/default file out…
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As a data steward responsible for managing and preserving of data generated in my lab, I want ensure raw data generated by a sequencing experiment (gzipped FastQ files, typically few GB per sample) is…
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Hello,
I am interested in direct-RNAseq data analysis. In the introduction, you have specified the used sequencing kit as "SQK-LSK108", which I believe is a cDNA or gDNA kit and not a dRNA kit. Cou…
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I am working on a project, which include RNA-seq data generated by total RNA library sequencing, with no specific filter for mRNA or enrichment for nascent RNA. Using IGV, my mapping bam files include…
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Hello!
Recently I've had to sequence and analyse a RNA-Seq set from T. cruzi RNA. For that, I used Salmon in the alignment-independent mode (aligning to a reference transcriptome).
Typical issues as…
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Discusses qPCR, RNA sequencing, single-cell RNA sequencing, and 16s rRNA Gene (DNA) sequencing.
# @grabear's Checklist
- [ ] Appropriate Content
- [ ] Well Formatted
- [ ] Working Links
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At the meeting last night, several people were interested in learning more about the biology underlying gene expression and RNA sequencing. The following links provide an introduction to these topics,…
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MINSEQE:
RNA-Seq (and others) should be changed to RNA-seq or cases need to match between applications.
sequencing_date is sequencing_data