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First off - thank you so much for continuing to respond to issues thus far. I'm trying to use your tool to stitch together a 3x2 microscopy image using the Nuclear stain channel, and following your "S…
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Hi,
I am a new suite2p user with very little python familiarity.
I am currently collecting a data set capturing live GCaMP6f activity in human brain organoids (imaged on a confocal microscope, not…
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What is the difference? When would I use one over the other?
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Dear Dr Dmitrieff,
I am sorry to disturb you.
in the search of a good tool for generating realistic confocal images, I stumbled across yours and Dr. Nedelecs program ConfocalGN. I managed to…
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When using the GAT feature in Fiji, it allows me to count the number of neurons in the image I upload, but it will not count the number of neurons per ganglia. After it counts the neurons, and I okay …
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I have confocal microscope images. I run this segmentation code "python -m cellpose --dir ~ --pretrained_model cyto --chan 2 --diameter 50 --save_png --no_npy --cellprob_threshold 0.0 --fast_mode".
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I am imaging large areas with a spinning disk confocal microscope and want to stitch the images with ashlar. Before stitching I would like to normalize the ranges in each image to a range between 0 an…
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Hello, i normally use libvips to process images from medical slides scanner (normally Hamamatsu) to dzi, however, i have new images from 3DHistech scanners and they produce the images as .dat files wi…
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- Histology - manually acquired, e.g. apotome or confocal
- Histology - brainsaw
- Histology - in situ sequencing
- MAPseq sequencing data and LCM images
- In vitro recordings
- Widefield overvie…
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In Sarah's experiments, we have the following problem: Sarah patches a neuron to record currents in a slice, then the slice is fixed, initial segments are labelled, and the slice is imaged under a con…