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### What kind of feature would you like to request?
Additional function parameters / changed functionality / changed defaults?
### Please describe your wishes
Hello,
I am trying to integrate som…
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**Describe the issue**
After pulling a SMART-seq3 unmapped bam, which is effectively after the initial demultiplexing step out of zUMIs (https://github.com/sdparekh/zUMIs/wiki/Protocol-specific-setup…
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I have been trying to run the nac workflow with the --tcc option enabled with the hopes of getting transcript level estimates for both nascent and mature transcripts.
The exact command that was run…
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Dear Sir/Madam,
Hope you are well.
Following resolved problem with the docker image on my cluster, I tried my first run with the launch_universc.sh with technology of STRT-Seq. I ran everything in …
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Hi,
Thanks for Velocyto, it's very interesting.
I try to launch "velocyto run" on our data.
Our scRNA-seq protocol is inspired by the STRT-Seq protocol. The results are in UMI counts and the re…
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Hi! First off, thanks for developing such a useful package. I have been running into some issues while trying to import data from TraCeR into anndata objects using scirpy following [the Scirpy tutoria…
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https://mp.weixin.qq.com/s/4JmnEOvb_d8TVdWpXDDizg
ixxmu updated
4 months ago
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hi,
went thru your wiki and your vignette page (https://satijalab.org/seurat/vignettes.html) but could not find a page that describes how to load data of various non-10x platforms into creating o…
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The `run10x` positional argument allows running trex on 10x Chromium data which have UMIs. Smartseq2 data don't have UMIs and for this purpose the `smartseq3` positional argument was added (the name o…
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Hi this is similar to https://github.com/satijalab/seurat/issues/3950 however despite my many attempts I cannot seem to get this to work with pac8.
```
library(Seurat)
library(SeuratData)
libra…