AnantharamanLab / METABOLIC

A scalable high-throughput metabolic and biogeochemical functional trait profiler
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Not a bug/ only a question #167

Open Sharifah89 opened 1 year ago

Sharifah89 commented 1 year ago

Dear AnantharamanLab team,

I read the article publised by your lab: Zhou, Z., St. John, E., Anantharaman, K. et al. Global patterns of diversity and metabolism of microbial communities in deep-sea hydrothermal vent deposits. Microbiome 10, 241 (2022). https://doi.org/10.1186/s40168-022-01424-7

I read that you used normalization before you conducted your analysis. I want to know if there is a command line to set the number of reads to a certain number. I have multiple samples I want to account for the depth of sequences and also will compare them to other datasets similar to your works. I hope I can get help regarding this simple yet crucial step.

"Screenshot 2023-10-07 at 2 33 26 PM"

source: Zhou, Z., St. John, E., Anantharaman, K. et al. Global patterns of diversity and metabolism of microbial communities in deep-sea hydrothermal vent deposits. Microbiome 10, 241 (2022). https://doi.org/10.1186/s40168-022-01424-7