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ArcInstitute / ScreenPro2

Flexible analysis of high-content CRISPR screening
https://screenpro2.rtfd.io
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Workflow for FACS-based screens #21

Closed abearab closed 5 months ago

abearab commented 7 months ago

root – https://github.com/GilbertLabUCSF/ScreenPro2/issues/17

abearab commented 7 months ago

For FACS based screens we usually sort two fluorescent bins and take the ratio to call phenotypes rather than comparing to the total population but both approaches are viable. Two papers talking about facs based screen design are linked below.