Closed yojetsharma closed 2 years ago
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You are using BAM files as input which require additional specification. Your error indicates that ArchR cannot find the information corresponding to the cell barcodes. Please see the BAM-related parameter documentation for createArrowFiles()
.
https://www.archrproject.com/reference/createArrowFiles.html
<simpleError: Detected 2 or less cells (0 barcodes have greater than 50 fragments) in file!
Check inputs such as 'minFrags' or 'maxFrags' to keep cells!
Also check that you are using the correct reference genome.
Exiting!
Worked! Thank you!
I am using my dataset for ArchR analyses. These are scATAC seq dataset. I am able to run through the initial steps of creation of arrow files but it seems to be stuck at "Creating ArrowFile From Temporary File 9.113 mins elapsed" for over two hours. And then once it finishes, it gives an empty character (0) for ArrowFiles.
ArchR-createArrows-3e077720032e-Date-2022-09-28_Time-11-16-36.log