Hello! I was interested in using your scripts and procedure for Mouse how did you make the "mature.miRNA.seqs.top50percent" file?

Gregor-Mendel-Institute / sRNA-spike-ins

Contains scripts used to design and analyze small RNA spike-in oligonucleotides as described in Lutzmayer, Enugutti and Nodine (2017) Novel small RNA spike-in oligonucleotides enable absolute normalization of small RNA-Seq data, Scientific Reports

2 stars 3 forks source link

Hello! I was interested in using your scripts and procedure for Mouse how did you make the "mature.miRNA.seqs.top50percent" file? #1

Open methornton opened 1 week ago

methornton commented 1 week ago

Hello!

Thank you for providing these scripts! I was wondering how you made the initial "mature.miRNA.seqs.top50percent" file for Arabidopsis and I was wondering if you could point me in the right direction to make a similar file for mouse with both piRNA and miRNA? Any advice is greatly appreciated.

Matt

methornton commented 1 week ago

Hello! I can get all of the mature mouse miRNA sequences from miRBase. I guess I can use them all and that should give me the random.fa that I would need? I'll give it a shot.