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GuyTeichman / RNAlysis

Analyze your RNA sequencing data without writing a single line of code
https://guyteichman.github.io/RNAlysis/
MIT License
129 stars 13 forks source link

PacBio sequencing and Transposons #17

Closed Ramkyeri closed 1 year ago

Ramkyeri commented 1 year ago

Dear GuyTecichman

Thank you so much for develpeing this tool. it is more easer firendly.

But I coud not find the running time, because I want to run more than 100 samples (Genome size is 2.5 Gb).

Can we use this tool to analysis long reds (PacBio sequencing) and Transposons from plant genome.

with regards

GuyTeichman commented 1 year ago

Hi Ramekyeri, Thank you for writing! I'm glad to hear you found this tool useful.

I'll assume that you meant long-read RNA sequencing (as opposed to genomic DNA). If that's the case, you should be able to quantify expression in your PacBio sequencing samples using kallisto (which is supported by RNAlysis), and all downstream analyses should be applicable to plant genomes as well.

Good luck with your analysis, Guy.