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HPCBio / 16S-rDNA-dada2-pipeline

**DEFUNCT** dada2-based Nextflow pipeline for multiple amplicon data. See website for up-to-date version
https://github.com/h3abionet/16S-rDNA-dada2-pipeline
MIT License
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Sanity check primers #23

Open cjfields opened 5 years ago

cjfields commented 5 years ago

One issue we should check is whether the primers used in the amplification match the 5' ends of the sequence. PI mentioned that some custom amplicons may not match up

cjfields commented 5 years ago

So far a spot check seems fine for the majority of data, but there are a few sequences where the sequence is a clear mismatch (likely misamps).

cjfields commented 5 years ago

Project-specific bug (low concentration sequence) and low-priority.