Closed noedelta closed 8 years ago
noedelta
commented
14 years ago Already present MI:0813 - but I will add PLA as a synonym. However, I'm not sure I'm happy where it is in the hierarchy - is this not a protein complementation assay?
Original comment by: orchard
noedelta
commented
13 years ago MI:0813 updated
Original comment by: orchard
noedelta
commented
13 years ago MI:0813 updated
Original comment by: orchard
noedelta
commented
13 years ago Original comment by: orchard
noedelta
commented
13 years ago Original comment by: orchard
noedelta
commented
12 years ago I'm also not happy where it sits - why is it a child of affinity technology (BTW - what was the original intention behind this term ? if one is to read the definition of MI:0400 - 'Techniques which depend upon the strength of the interaction between two entities.' it sounds as every technique is affinitiy technique ?) it seems to be closer to any technique providing information that two (or more) molecules are nearby. shouldn't it be, at least, moved one level up ?
as far as the name goes - shuoldn't it be just 'proximity ligation assay' as ligation of two peices of DNA brought together in space is the key feature of this method ?
the definition of this term curantly states: 'Method allowing efficient and precise interaction detection, along with extensive repertoires of specific binding reagents. It is based on proximity a ligation mechanism that enables sensitive high-capacity protein measurements by converting the detection of specific proteins to the analysis of DNA sequences. Proximity probes containing oligonucleotide extensions are designed to bind pairwise to target proteins and to form amplifiable tag sequences by ligation when brought in proximity.'
It is quite long and sounds somewhat awkward - what about shortening it to ' A method bases on ligation of two pieces of DNA brought together in space by interacting molecules. Proximity probes containing oligonucleotide extensions are typically designed as antibodies binding to the target interacting proteins.'
Original comment by: lukasz99
noedelta
commented
12 years ago Original comment by: lukasz99
noedelta
commented
11 years ago OK, name is now 'proximity ligation assay' and all references to p-elisa have been retired as synonyms - I have no idea where Luisa got that name from as I can't find it used anywhere. Definition also updated.
Original comment by: orchard
noedelta
commented
11 years ago Original comment by: orchard
Can we add “in situ proximity ligation assay” as a method to detect protein-protein interaction? This technique has been described in PMID 17072308: “Proximity probes-oligonucleotides attached to antibodies against the two target proteins-guided the formation of circular DNA strands when bound in close proximity. The DNA circles in turn served as templates for localized rolling-circle amplification (RCA), allowing individual interacting pairs of protein molecules to be visualized and counted in human cell lines and clinical specimens.”
Reported by: lperfetto