noedelta
commented
18 years ago Logged In: YES user_id=653048
This is an alternative to the request 1465006 from Lukasz : EITHER we report the fusion protein used as features thus it would be redundant to have specific methods according to the specific DNA-DB and DNA-AD OR we create a method for the major combination of fusion proteins specifying which is attached to the bait and which to the prey and then avoid to repeat the specific fusion of each interactor.
Consider that Mappit (MI:0231) and ubiquitin split (MI:0112) are already in the CV. And certainly LUMIER should be added in any case.
Original comment by: luisa_montecchi
1-Gal4 transcription regeneration Stan Fields powerful genetic selections Chien CT, Bartel PL, Sternglanz R, Fields S The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9578-82 1946372
2-Gal4 transcription regeneration Marc Vidal genetic markers are reversed; selection against an interaction is possible Vidal M, Brachmann RK, Fattaey A, Harlow E, Boeke JD Reverse two-hybrid and one-hybrid systems to detect dissociation of protein-protein and DNA-protein interactions. Proc Natl Acad Sci U S A. 1996 Sep 17;93(19):10315-20 8816797
3-LexA/B52 transcription regeneration Roger Brent LexA DB eplaces Gal4 DB; B52 acidic seq replaces GAL4 AD Zhong J, Zhang H, Stanyon CA, Tromp G, Finley RL Jr A strategy for constructing large protein interaction maps using the yeast two-hybrid system: regulated expression arrays and two-phase mating. Genome Res 2003, 13:2691-2699 14613974
4-mammalian two-hybrid Eric Fearon Gal4 BD; VP16 AD; luciferase reporter Fearon ER, Finkel T, Gillison ML, Kennedy SP, Casella JF, Tomaselli GF, Morrow JS, Van Dang C. Karyoplasmic interaction selection strategy: A general strategy to detect protein-protein interactions in mammalian cells Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):7958-62 1387709
5-bacterial two-hybrid Daniel Ladant cAMP signaling pathway reconstitution; genetic selection Karimova G, Pidoux J, Ullmann A, Ladant D A bacterial two-hybrid system based on a reconstituted signal transduction pathway Proc Natl Acad Sci U S A. 1998 May 12;95(10):5752-6. 9576956
6- split ubiquitin Wolf Frommer reconstitution of N-ter Nub & C-ter Cub ubiquitin halves releases LexA-VP16 transcription factor Obrdlik P, El-Bakkoury M, Hamacher T, Cappellaro C, Vilarino C, Fleischer C, Ellerbrok H, Kamuzinzi R, Ledent V, Blaudez D, Sanders D, Revuelta JL, Boles E, Andre B, Frommer WB. K+ channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions Proc Natl Acad Sci U S A. 2004 Aug 17;101(33):12242-7 15299147
6- LUMIER luminescence-based mammalian interactome mapping Jeff Wrana pulldown NOT genetic interaction strategy, therefore not really two-hybrid Barrios-Rodiles M, Brown KR, Ozdamar B, Bose R, Liu Z, Donovan RS, Shinjo F, Liu Y, Dembowy J, Taylor IW, Luga V, Przulj N, Robinson M, Suzuki H, Hayashizaki Y, Jurisica I, Wrana JL High-throughput mapping of a dynamic signaling network in mammalian cells Science. 2005 Mar 11;307(5715):1621-5 15761153
7-MAPPIT mammalian protein-protein interaction trap
Reported by: luisa_montecchi