电子细菌宠物

[Jackxiaoyuchen]

因为道长跟我说，要是能够用酵母人工合成大麻，会很酷炫。当时我就在想了想，其实抛去需要的基因组(synthetic gene circuits)我觉得技术难点对我们来说，应该是DIY bioreactor + directed evolution的一套系统。那么我以前帝国的一个老师，Mark Isalan 做过一个很酷的利用Bacteria Phage 来达到optimal protein interaction 的这样一组directed evolution系统。但是他们的系统是公司给做的，然后我又看到了用arduino bioreactor的一个paper （还没review），这个idea就油然而生

如何做一个电子宠物，让大家都简单的设定几个条件然后反应堆自行解答给你（我想要产大麻素最牛逼的酵母etc ) ？

bioreactor https://makezine.com/2016/07/18/arduino-powered-bioreactors-make-home-experimentation-affordable/

https://www.hackster.io/open-bioeconomy-lab/microbial-bioreactor-d7f61b

https://peerj.com/preprints/27150.pdf

Mark Isalan的google page以及他发的natureprotocol （ 注意，是protocol，手把手告诉你怎么做）

https://www.imperial.ac.uk/people/m.isalan/publications.html

https://www.sciencedirect.com/science/article/pii/S0958166917301350?via%3Dihub https://www.nature.com/articles/nprot.2017.084

[shouldsee]

How hard is it to devicify directed evolution? Seems one would definitely requires microfluidic for this purpose?

[Jackxiaoyuchen]

So far, I guess the step mainly involved 1) bioreactor 2) liquid handling robotics 3) optics assay

Seed the cells with mutant library (or mutator strains)

Choosing a target marker ( high yield, fast growing speed etc)

Push pressure (toxin antidote, phage infection, or auxotrophic mutant)

liquid handling part to screen ( high yield can be linked to GFP or better growth fitness OD)

Select and store

Sequencing to identify the changes.

[Jackxiaoyuchen]

https://www.culturebiosciences.com/

你可以看看这个，我给道长看过了，一个india的公司

[shouldsee]

Select and change is probably the hardest part, since we need to pipe the results back in as a new mutant library.

[Jackxiaoyuchen]

Off, the liquid handling is hard. However, like I point out in the paper, the phage pressured method allow you to have one uniform batch ( all strains are the same, the best man win) we can just store them to a reserving pot after each batch completed, then start with this and grow it up ( with plasmid of mutant library )

This one, not manual handling is required.

Best,

Jack xiaoyu chen DPhil in Engineering Science University of Oxford Beg broke Science Park Oxford United Kingdom TEL: +44(0)7731516944

On 18 Apr 2019, at 20:33, shouldsee notifications@github.com wrote:

Select and change is probably the hardest part, since we need to pipe the results back in as a new mutant library.

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[Jackxiaoyuchen]

https://www.labgeni.us/

这次开会，就这个公司，你把你的菌株给他们，他们来给你优化。然后远程宠物，慢慢养，养好给你。酵母菌托儿所