search

Jiaxin-Fan / ASEP

8 stars 3 forks source link

raw allele-specific read count or normalized count? #4

Open ghost opened 3 years ago

ghost commented 3 years ago

Hi,

I want to ask about the input read count value? Should I simply give the raw allele-specific read count? Or should I normalize (for library size differences) the raw count by myself and then run for ASE?

I would presume that the likelihood method doesn't need the count be normally distributed, so the quantile normalization would be skipped for this purpose, right?

Thanks for your help!

Meiyuan

Jiaxin-Fan commented 3 years ago

Hi Meiyuan,

The input use the raw allelic count. Yes, no need to normalize.

Best, Jiaxin