I want to ask about the input read count value? Should I simply give the raw allele-specific read count? Or should I normalize (for library size differences) the raw count by myself and then run for ASE?
I would presume that the likelihood method doesn't need the count be normally distributed, so the quantile normalization would be skipped for this purpose, right?
Hi,
I want to ask about the input read count value? Should I simply give the raw allele-specific read count? Or should I normalize (for library size differences) the raw count by myself and then run for ASE?
I would presume that the likelihood method doesn't need the count be normally distributed, so the quantile normalization would be skipped for this purpose, right?
Thanks for your help!
Meiyuan