search

MaestSi / MetONTIIME

A Meta-barcoding pipeline for analysing ONT data in QIIME2 framework
GNU General Public License v3.0
78 stars 17 forks source link

deprepSeq not created #86

Closed ObRICa closed 9 months ago

ObRICa commented 9 months ago

I have encountered by this error. I could not figure out what is wrong, since the error continued even after preparing a proper metadata (and its link in the config file). There were 2 files created in the derepSeq folder, rep-seqs.qza and table.qza. I tried to feature summarize this table, however it was failed with the message that there is a problem in the file format. I think that the process was not completed there. The same error occurred even when I ran the process with only one fastq.gz. I would appreciate your help.

Here is the log:

Command exit status: 1

Command output: Saved FeatureTable[Frequency] to: /Users/xx/nextflow_MetONTIIME/MetONTIIME/2023GM/resultDir/derepSeq/table_tmp.qza Saved FeatureData[Sequence] to: /Users/xx/nextflow_MetONTIIME/MetONTIIME/2023GM/resultDir/derepSeq/rep-seqs_tmp.qza Saved FeatureTable[Frequency] to: /Users/xx/nextflow_MetONTIIME/MetONTIIME/2023GM/resultDir/derepSeq/table.qza Saved FeatureData[Sequence] to: /Users/xx/nextflow_MetONTIIME/MetONTIIME/2023GM/resultDir/derepSeq/rep-seqs.qza

Command error: Saved FeatureTable[Frequency] to: /Users/xx/nextflow_MetONTIIME/MetONTIIME/2023GM/resultDir/derepSeq/table_tmp.qza Saved FeatureData[Sequence] to: /Users/xx/nextflow_MetONTIIME/MetONTIIME/2023GM/resultDir/derepSeq/rep-seqs_tmp.qza Saved FeatureTable[Frequency] to: /Users/xx/nextflow_MetONTIIME/MetONTIIME/2023GM/resultDir/derepSeq/table.qza Saved FeatureData[Sequence] to: /Users/xx/nextflow_MetONTIIME/MetONTIIME/2023GM/resultDir/derepSeq/rep-seqs.qza Plugin error from feature-table:

The following IDs are not present in the metadata: 'BC01', 'BC02', 'BC03', 'BC04', 'BC05', 'BC06', 'BC07', 'BC08', 'BC09', 'BC10', 'BC11', 'BC12', 'BC13', 'BC14', 'BC15', 'BC16', 'BC17', 'BC18', 'BC19', 'BC20', 'BC21', 'BC22', 'BC23', 'BC24'
ObRICa commented 9 months ago

I think I found the solution! Please delete this issue. Thank you.