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MahShaaban / pcr

Quality assessing, analyzing and testing the statistical significance of real-time quantitative PCR data
https://CRAN.R-project.org/package=pcr
GNU General Public License v3.0
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CpAverage or CrossingPoint #25

Closed lmanchon closed 3 years ago

lmanchon commented 3 years ago

--Hi all,

I have a question about the output ixo files from the Lightcycler 480: in these files there are the CpAverage and CrossingPoint values. What values do I need to retrieve to use the pcr package?

Thanks

MahShaaban commented 3 years ago

Hi @lmanchon I am not familiar with this particular machine. However, I think, it's the cp Average you should use. If you could provide an example file from the machine output, I might be able to confirm. Cheers

lmanchon commented 3 years ago

--Hi,

ok you can find an example in attachment here. thanks. TNFa.ixo.gz

MahShaaban commented 3 years ago

I can't open .ixo files on my machine. Is there a way to export the data into .txt files? If no, you could copy the relevant parts into a text file and share it.

lmanchon commented 3 years ago

-- ixo file is just a text file. You can open it with notepad+ on Windows machine or just with vim or vi on Linux system.

MahShaaban commented 3 years ago

Yes. I can. I am not sure however which of lines are relevant to the question.

lmanchon commented 3 years ago

-- you can use grep to catch the lines with "CpAverage" or "CrossingPoint". eaF1 is the gene control.