AMR++ is a bioinformatic pipeline meant to aid in the analysis of raw sequencing reads to characterize the profile of antimicrobial resistance genes, or resistome.
we used your pipeline for some resistome analysis on complex samples and we were wondering how the Hits are calculated within the pipeline? As we couldn't figure it out from the source code, we would like to know if the same sequence can have multiple hits to the reference DB and how this would be counted? Or did the pipeline just count the best reference hit for each sequence?
Dear Microbial Ecology Group,
we used your pipeline for some resistome analysis on complex samples and we were wondering how the Hits are calculated within the pipeline? As we couldn't figure it out from the source code, we would like to know if the same sequence can have multiple hits to the reference DB and how this would be counted? Or did the pipeline just count the best reference hit for each sequence?
Thanks for guiding us on this!
Best, Markus