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MiraldiLab / maxATAC

Transcription Factor Binding Prediction from ATAC-seq and scATAC-seq with Deep Neural Networks
Apache License 2.0
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bedGraphToBigWig: error while loading shared libraries: libssl.so.1.0.0: cannot open shared object file: No such file or directory #102

Closed tacazares closed 1 year ago

tacazares commented 2 years ago

@anthonybejjani had an issue with running maxatac prepare where bedGraphToBigWig was not recognized. He was on an LSF system using anaconda.

I followed the install instructions for maxATAC and was able to reproduce this error. 

bedGraphToBigWig K562_IS_slop20_RP20M.bg hg38.chrom.sizes K562_IS_slop20_RP20M.bw
bedGraphToBigWig: error while loading shared libraries: libssl.so.1.0.0: cannot open shared object file: No such file or directory

I did not have this issue when installing on my local MAC machine.

tacazares commented 2 years ago

I was able to fix this error using three approaches:

1) If your HPC system has bedGraphToBigWig installed, then do not install it with conda during maxatac installation. Load it through your HPC environment instead.

2) Reinstall bedGraphToBigWig and make sure you symlink the command to the correct path for your conda installation in the ./opt/ directory.

3) I was able to also activate my conda env and then load the module available on my HPC. This corrected the error.

I think there is an error with the installation and the paths that it searches for bedGraphToBigWig.