Making this issue as an overview of the discussion.
The illumination control describes a (LabView) feedback loop that keeps the mean brightness (intensity) of the image, as calculated and returned by the BeadTracker, at a constant level. It would require a LabView-controlled power source to crank up the LED intensity.
The first motivation was that keeping the illumination (S/N ratio) constant would cause a constant accuracy throughout the measurement. However, data analysis tells us that the S/N ratio is not a limiting factor due to QI converging at nearly the same rate after a threshold S/N ratio is reached. However, it might influence the accuracy of the ZLUT and the robustness of background correction. (This part has been discussed with Jacob Kerssemakers).
In a first meeting with Belen, we have decided not to pursue this functionality currently. However, this might change in the future. There is also a document prepared by Jordi on this issue.
Making this issue as an overview of the discussion.
The illumination control describes a (LabView) feedback loop that keeps the mean brightness (intensity) of the image, as calculated and returned by the BeadTracker, at a constant level. It would require a LabView-controlled power source to crank up the LED intensity.
The first motivation was that keeping the illumination (S/N ratio) constant would cause a constant accuracy throughout the measurement. However, data analysis tells us that the S/N ratio is not a limiting factor due to QI converging at nearly the same rate after a threshold S/N ratio is reached. However, it might influence the accuracy of the ZLUT and the robustness of background correction. (This part has been discussed with Jacob Kerssemakers).
In a first meeting with Belen, we have decided not to pursue this functionality currently. However, this might change in the future. There is also a document prepared by Jordi on this issue.