loss of protein marker names and corruption of files following normalization

ParkerICI / premessa

R package for pre-processing of mass and flow cytometry data

GNU General Public License v3.0

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loss of protein marker names and corruption of files following normalization #21

Closed CNicholasMDA closed 5 years ago

CNicholasMDA commented 5 years ago

Hello, I am suddenly having trouble normalizing and debarcoding files properly using premessa. Following normalization, I lose all protein marker names associated with each metal channel, and the resulting files do not look normal when placed in FlowJo.

Can anyone suggest a troubleshooting method? I am concerned that one of the supporting packages has changed but I do not know how to diagnose it. Thank you, Courtney

pfgherardini commented 5 years ago

Hi Courtney,

Sorry for the late reply. Are you still having problems? Can you please share an example file with me?

Thanks

Federico

CNicholasMDA commented 5 years ago

Federico, Thanks for checking back in. Apologies because I never closed this thread. It looks like for now my issue has resolved. Must've been a temporary issue. Thanks! -Courtney