Stitching Time lapse movie: unexpected results in Calculate Pairwise Shifts

[walkernoreen]

Hello,

The calculation of pairwise shifts for a time lapse movie seems to show inconsistent behaviour.

I have a movie (12 tiles per time point (each tile a zstack), 70 time points) and stitch them in two ways, in both cases each time point individually.

(1) Processing the whole movie at once, with 'each time point individually' checked. This does not work. Many correlations are calculated, but they seem to be calculated between random tile pairs. The interactive link previewer misses many links. Settings used: BigStitcher GUI: Select all tiles -> Calculate Pairwise Shifts ... -> Phase Correlation (expert) -> chek "show expert grouping options" -> Select Views to Process: TimePoints: all, Tiles: all -> Select how to Process views: TimePoints: individually, Tiles: compare (The same issue happens when going through batch processing)

(2) Scripting and processing single time points at a time This works. But in my understanding this should produce the same results as (1). Settings used: record this action:

• BigStitcher Batch processing -> Calculate Pairwise Shifts
• Process tile: All tiles, process timepoint: single time points [select from list]
• Grouping: as above

then loop over time points with a python script.

[hoerldavid]

Hi @walkernoreen ,

Sorry for taking so long to catch up here. That is indeed unexpected behavior - you were using it as intended. I'll look into the issue and let you know if I can reproduce the problem - otherwise, would you be willing to share images from ~2 timepoints, so we can test your problem?

Best, David