Open kaur-a2023 opened 1 year ago
I have been trying to design Indel markers for KASP genotyping for wheat through web-based Polymarker. My file looks something like this:
UI59,7D,ATGCCACACTTATAAAATTATCTTCTACACACTTGCTTAGGATCAAATCAAGCATCATGTCGTGTACTGTGCAACACAACACCTCTCCATACTCGATTTCC[G/-]ATGGCTGAATCATGCTTCTGTTGACGAGCTCATTGAAATAACTCCTGCCAACATCCTCCAAATCTGGCCCATGCAAACTGCTGACGAAGCCTTCAGCTA UI822,7D,CGTACATGTTACCGAACGCCACCGTGCCGACGAAGCCGTCCATGTACCCGAAGACGTGATCCTCCAGACATACTGGTCCCTGTCCATTAACGCTAGCTACT[-/A]GCTAGTCCGCTCACCAGTTTACCCACCTCAGCCTCCCTGGCATACCAGGTGGCCTGGATGCGGCGCTTGCTGAGGAACTCGACGACCAGGAGCTTGCGCC UI456,7D,GGGGTTGCGCCGTCGCCGGAGACACCAGCAAAGTTGTGGCGTCGCGCGTTGTTGCTGTAGGTCGTGTAGAGGAAGCCGGCGAGGGTTTGGGGCAGCTTCTT[GC/-]GGTTGGCGGGGTCGGCGATGAGGTCGCGCCAGCGCACGGAGACGCACTTGAAGCGGTGGAGGGATCTGGCGGGGAGGCGGGAGAGGATCTCCAGGATG
But I am getting the following error:
No such file or directory @ rb_sysopen - /opt/data/tmp//64c2965fd31c6f47d956b0f6_out/to_align.fa.fai
I am not sure if Polymarker cannot be used for InDels or its some kind of issue with my file.
Could you please help me out with the issue. Thanks.
I have been trying to design Indel markers for KASP genotyping for wheat through web-based Polymarker. My file looks something like this:
But I am getting the following error:
No such file or directory @ rb_sysopen - /opt/data/tmp//64c2965fd31c6f47d956b0f6_out/to_align.fa.fai
I am not sure if Polymarker cannot be used for InDels or its some kind of issue with my file.
Could you please help me out with the issue. Thanks.