Hi.I'm trying to use MINI-EX,but It report an error
N E X T F L O W ~ version 22.10.0 Launchingminiex.nf` [intergalactic_perlman] DSL2 - revision: 83499324ee
Motif-Informed Network Inference from gene EXpression
Running TF motif enrichment filtering on TF-F_motifs
Running single-cell cluster enrichment using the top 700 upregulated genes per cluster
Filtering out regulons of single-cell clusters where the TF is expressed in less than 10 % of the cells
Plotting expression specificity and DE calls for the top 150 regulons
[- ] process > run_grnboost -
[- ] process > unzip_motifMappings -
[- ] process > run_enricher_motifs -
[- ] process > filter_motifs -
[- ] process > get_topDEGs -
[- ] process > run_enricher_cluster -
[- ] process > filter_expression -
[- ] process > make_info_file -
[- ] process > clustermap_regs -
[- ] process > network_centrality -
[- ] process > getFiles_enrichment -
[- ] process > GO_enricher -
[- ] process > check_reference -
[- ] process > ranking_df_ref -
[- ] process > makeBorda -
[- ] process > heatmap_tops -
WARN: The echo directive has been deprecated - use to debug instead
[- ] process > run_grnboost [ 0%] 0 of 1
[- ] process > unzip_motifMappings [ 0%] 0 of 1
[- ] process > run_enricher_motifs -
[- ] process > filter_motifs -
[- ] process > get_topDEGs [ 0%] 0 of 1
[- ] process > run_enricher_cluster -
[- ] process > filter_expression -
[- ] process > make_info_file -
[- ] process > clustermap_regs -
[- ] process > network_centrality -
[- ] process > getFiles_enrichment -
[- ] process > GO_enricher -
[- ] process > check_reference -
[- ] process > ranking_df_ref -
[- ] process > makeBorda -
[- ] process > heatmap_tops -
[miniexExample, /public1/home/scb0721/sc52142/hq/MINI-EX-main/example/INPUTS/miniexExample_matrix.txt]
WARN: The echo directive has been deprecated - use to debug instead
[- ] process > run_grnboost [ 0%] 0 of 1
[- ] process > unzip_motifMappings [ 0%] 0 of 1
[- ] process > run_enricher_motifs -
[- ] process > filter_motifs -
[- ] process > get_topDEGs [ 0%] 0 of 1
[- ] process > run_enricher_cluster -
[- ] process > filter_expression -
[- ] process > make_info_file -
[- ] process > clustermap_regs -
[- ] process > network_centrality -
[- ] process > getFiles_enrichment -
[- ] process > GO_enricher -
[- ] process > check_reference -
[- ] process > ranking_df_ref -
[- ] process > makeBorda -
[- ] process > heatmap_tops -
[miniexExample, /public1/home/scb0721/sc52142/hq/MINI-EX-main/example/INPUTS/miniexExample_matrix.txt]
WARN: The echo directive has been deprecated - use to debug instead
Error executing process > 'run_grnboost (1)'
Caused by:
Failed to submit process to grid scheduler for execution
Hi.I'm trying to use MINI-EX,but It report an error
N E X T F L O W ~ version 22.10.0 Launching
miniex.nf` [intergalactic_perlman] DSL2 - revision: 83499324ee Motif-Informed Network Inference from gene EXpressionRunning TF motif enrichment filtering on TF-F_motifs Running single-cell cluster enrichment using the top 700 upregulated genes per cluster Filtering out regulons of single-cell clusters where the TF is expressed in less than 10 % of the cells Plotting expression specificity and DE calls for the top 150 regulons
[- ] process > run_grnboost - [- ] process > unzip_motifMappings - [- ] process > run_enricher_motifs - [- ] process > filter_motifs - [- ] process > get_topDEGs - [- ] process > run_enricher_cluster - [- ] process > filter_expression - [- ] process > make_info_file - [- ] process > clustermap_regs - [- ] process > network_centrality - [- ] process > getFiles_enrichment - [- ] process > GO_enricher - [- ] process > check_reference - [- ] process > ranking_df_ref - [- ] process > makeBorda - [- ] process > heatmap_tops - WARN: The
echo
directive has been deprecated - use todebug
instead[- ] process > run_grnboost [ 0%] 0 of 1 [- ] process > unzip_motifMappings [ 0%] 0 of 1 [- ] process > run_enricher_motifs - [- ] process > filter_motifs - [- ] process > get_topDEGs [ 0%] 0 of 1 [- ] process > run_enricher_cluster - [- ] process > filter_expression - [- ] process > make_info_file - [- ] process > clustermap_regs - [- ] process > network_centrality - [- ] process > getFiles_enrichment - [- ] process > GO_enricher - [- ] process > check_reference - [- ] process > ranking_df_ref - [- ] process > makeBorda - [- ] process > heatmap_tops - [miniexExample, /public1/home/scb0721/sc52142/hq/MINI-EX-main/example/INPUTS/miniexExample_matrix.txt] WARN: The
echo
directive has been deprecated - use todebug
instead[- ] process > run_grnboost [ 0%] 0 of 1 [- ] process > unzip_motifMappings [ 0%] 0 of 1 [- ] process > run_enricher_motifs - [- ] process > filter_motifs - [- ] process > get_topDEGs [ 0%] 0 of 1 [- ] process > run_enricher_cluster - [- ] process > filter_expression - [- ] process > make_info_file - [- ] process > clustermap_regs - [- ] process > network_centrality - [- ] process > getFiles_enrichment - [- ] process > GO_enricher - [- ] process > check_reference - [- ] process > ranking_df_ref - [- ] process > makeBorda - [- ] process > heatmap_tops - [miniexExample, /public1/home/scb0721/sc52142/hq/MINI-EX-main/example/INPUTS/miniexExample_matrix.txt] WARN: The
echo
directive has been deprecated - use todebug
instead Error executing process > 'run_grnboost (1)'Caused by: Failed to submit process to grid scheduler for execution
Command executed:
qsub -terse .command.run
Command exit status: 2
Command output: Unknown option: terse Usage: qsub [-a start_time] [-A account] [-b y|n] [-e err_path] [-I] [-l resource_list] [-m mail_options] [-M user_list] [-N job_name] [-o out_path] [-p priority] [-pe shm task_cnt] [-P wckey] [-q destination] [-r y|n] [-v variable_list] [-V] [-wd workdir] [-W additional_attributes] [-h] [script]
Work dir: /public1/home/scb0721/sc52142/hq/MINI-EX-main/work/a3/dcbbbccbc942696df1e150727f2153
Tip: when you have fixed the problem you can continue the execution adding the option
-resume
to the run command lineOops .. something went wrong
[a3/dcbbbc] process > run_grnboost (1) [100%] 1 of 1, failed: 1 [- ] process > unzip_motifMappings [ 0%] 0 of 1 [- ] process > run_enricher_motifs - [- ] process > filter_motifs - [- ] process > get_topDEGs [ 0%] 0 of 1 [- ] process > run_enricher_cluster - [- ] process > filter_expression - [- ] process > make_info_file - [- ] process > clustermap_regs - [- ] process > network_centrality - [- ] process > getFiles_enrichment - [- ] process > GO_enricher - [- ] process > check_reference - [- ] process > ranking_df_ref - [- ] process > makeBorda - [- ] process > heatmap_tops - [miniexExample, /public1/home/scb0721/sc52142/hq/MINI-EX-main/example/INPUTS/miniexExample_matrix.txt] WARN: The
echo
directive has been deprecated - use todebug
instead Error executing process > 'run_grnboost (1)'Caused by: Failed to submit process to grid scheduler for execution
Command executed:
qsub -terse .command.run
Command exit status: 2
Command output: Unknown option: terse Usage: qsub [-a start_time] [-A account] [-b y|n] [-e err_path] [-I] [-l resource_list] [-m mail_options] [-M user_list] [-N job_name] [-o out_path] [-p priority] [-pe shm task_cnt] [-P wckey] [-q destination] [-r y|n] [-v variable_list] [-V] [-wd workdir] [-W additional_attributes] [-h] [script]
Work dir: /public1/home/scb0721/sc52142/hq/MINI-EX-main/work/a3/dcbbbccbc942696df1e150727f2153
Tip: when you have fixed the problem you can continue the execution adding the option
-resume
to the run command line` How do I solve it?