VIB-PSB / MINI-EX

Motif-Informed Network Inference of cell type-specific gene regulatory networks in plants
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Error executing process > 'run_grnboost (1)' #6

Closed Huo-qiang closed 1 year ago

Huo-qiang commented 2 years ago

Hi.I'm trying to use MINI-EX,but It report an error N E X T F L O W ~ version 22.10.0 Launchingminiex.nf` [intergalactic_perlman] DSL2 - revision: 83499324ee Motif-Informed Network Inference from gene EXpression

Running TF motif enrichment filtering on TF-F_motifs Running single-cell cluster enrichment using the top 700 upregulated genes per cluster Filtering out regulons of single-cell clusters where the TF is expressed in less than 10 % of the cells Plotting expression specificity and DE calls for the top 150 regulons

[- ] process > run_grnboost - [- ] process > unzip_motifMappings - [- ] process > run_enricher_motifs - [- ] process > filter_motifs - [- ] process > get_topDEGs - [- ] process > run_enricher_cluster - [- ] process > filter_expression - [- ] process > make_info_file - [- ] process > clustermap_regs - [- ] process > network_centrality - [- ] process > getFiles_enrichment - [- ] process > GO_enricher - [- ] process > check_reference - [- ] process > ranking_df_ref - [- ] process > makeBorda - [- ] process > heatmap_tops - WARN: The echo directive has been deprecated - use to debug instead

[- ] process > run_grnboost [ 0%] 0 of 1 [- ] process > unzip_motifMappings [ 0%] 0 of 1 [- ] process > run_enricher_motifs - [- ] process > filter_motifs - [- ] process > get_topDEGs [ 0%] 0 of 1 [- ] process > run_enricher_cluster - [- ] process > filter_expression - [- ] process > make_info_file - [- ] process > clustermap_regs - [- ] process > network_centrality - [- ] process > getFiles_enrichment - [- ] process > GO_enricher - [- ] process > check_reference - [- ] process > ranking_df_ref - [- ] process > makeBorda - [- ] process > heatmap_tops - [miniexExample, /public1/home/scb0721/sc52142/hq/MINI-EX-main/example/INPUTS/miniexExample_matrix.txt] WARN: The echo directive has been deprecated - use to debug instead

[- ] process > run_grnboost [ 0%] 0 of 1 [- ] process > unzip_motifMappings [ 0%] 0 of 1 [- ] process > run_enricher_motifs - [- ] process > filter_motifs - [- ] process > get_topDEGs [ 0%] 0 of 1 [- ] process > run_enricher_cluster - [- ] process > filter_expression - [- ] process > make_info_file - [- ] process > clustermap_regs - [- ] process > network_centrality - [- ] process > getFiles_enrichment - [- ] process > GO_enricher - [- ] process > check_reference - [- ] process > ranking_df_ref - [- ] process > makeBorda - [- ] process > heatmap_tops - [miniexExample, /public1/home/scb0721/sc52142/hq/MINI-EX-main/example/INPUTS/miniexExample_matrix.txt] WARN: The echo directive has been deprecated - use to debug instead Error executing process > 'run_grnboost (1)'

Caused by: Failed to submit process to grid scheduler for execution

Command executed:

qsub -terse .command.run

Command exit status: 2

Command output: Unknown option: terse Usage: qsub [-a start_time] [-A account] [-b y|n] [-e err_path] [-I] [-l resource_list] [-m mail_options] [-M user_list] [-N job_name] [-o out_path] [-p priority] [-pe shm task_cnt] [-P wckey] [-q destination] [-r y|n] [-v variable_list] [-V] [-wd workdir] [-W additional_attributes] [-h] [script]

Work dir: /public1/home/scb0721/sc52142/hq/MINI-EX-main/work/a3/dcbbbccbc942696df1e150727f2153

Tip: when you have fixed the problem you can continue the execution adding the option -resume to the run command line

Oops .. something went wrong

[a3/dcbbbc] process > run_grnboost (1) [100%] 1 of 1, failed: 1 [- ] process > unzip_motifMappings [ 0%] 0 of 1 [- ] process > run_enricher_motifs - [- ] process > filter_motifs - [- ] process > get_topDEGs [ 0%] 0 of 1 [- ] process > run_enricher_cluster - [- ] process > filter_expression - [- ] process > make_info_file - [- ] process > clustermap_regs - [- ] process > network_centrality - [- ] process > getFiles_enrichment - [- ] process > GO_enricher - [- ] process > check_reference - [- ] process > ranking_df_ref - [- ] process > makeBorda - [- ] process > heatmap_tops - [miniexExample, /public1/home/scb0721/sc52142/hq/MINI-EX-main/example/INPUTS/miniexExample_matrix.txt] WARN: The echo directive has been deprecated - use to debug instead Error executing process > 'run_grnboost (1)'

Caused by: Failed to submit process to grid scheduler for execution

Command executed:

qsub -terse .command.run

Command exit status: 2

Command output: Unknown option: terse Usage: qsub [-a start_time] [-A account] [-b y|n] [-e err_path] [-I] [-l resource_list] [-m mail_options] [-M user_list] [-N job_name] [-o out_path] [-p priority] [-pe shm task_cnt] [-P wckey] [-q destination] [-r y|n] [-v variable_list] [-V] [-wd workdir] [-W additional_attributes] [-h] [script]

Work dir: /public1/home/scb0721/sc52142/hq/MINI-EX-main/work/a3/dcbbbccbc942696df1e150727f2153

Tip: when you have fixed the problem you can continue the execution adding the option -resume to the run command line

` How do I solve it?