YeoLab / MINES

(m)6A (I)dentification Using (N)anopor(E) (S)equencing
18 stars 3 forks source link

what is the coverage means? #23

Open JXAUDZF opened 6 months ago

JXAUDZF commented 6 months ago

I measured the m6A site values of 46 samples, but I don't understand the significance of coverage in each sample. Is it the number of reads or the peak value of m6A sites at each site? Can coverage be directly considered as the m6A peak value of each sample grade point? Is the peak value between 46 samples coverage

JXAUDZF commented 6 months ago

which value represent the value of m6A site peak

JXAUDZF commented 6 months ago

Can the coverage value represent the modification kurtosis if the sequencing depth of 46 samples is different?

JXAUDZF commented 6 months ago

Which value represents the level of m6A modification

JXAUDZF commented 6 months ago

Is the coverage value the standardized result of coverage for each sample read