Error in round(result$tail_length, digits = 2)

[jchang97]

Thank you so much for this amazing tool!

Just wanted to ask about an error I get while processing a nanopore dRNA fast5 file:

────────────────────────────────────────────────────────────────────────────────
── Started tailfindr (version 1.3) ─────────────────────────────────────────────
────────────────────────────────────────────────────────────────────────────────
☰ You have configured tailfindr as following:
❯ fast5_dir:         /data/gpfs/projects/punim1068/data/direct_RNA/Calu/Calu_24hpi_I_1_fast5/output/fast5/workspace/fast5_skip/0
❯ save_dir:          /data/gpfs/projects/punim1068/data/direct_RNA/Calu/Calu_24hpi_I_1_fast5/output/fast5/workspace/fast5_skip/0
❯ csv_filename:      tails.csv
❯ num_cores:         4
❯ basecall_group:    Basecall_1D_001
❯ save_plots:        FALSE
❯ plot_debug_traces: FALSE
❯ plotting_library:  rbokeh
── Processing started at 2022-09-26 08:12:02 ───────────────────────────────────
• Searching for all Fast5 files...
  Done! Found 1 Fast5 files.
• Analyzing a single Fast5 file to assess if your data 
  is in an acceptable format...
  ✔ The data has been basecalled using Guppy.
  ✔ Flipflop model was used during basecalling.
  ✔ Every read is in a single fast5 file of its own.
  ✔ The experiment type is RNA, so we will search
    for poly(A) tails.
  ✔ The reads are 1D reads.
• Starting a parallel compute cluster...
  Done!
• Searching for Poly(A) tails...
  Processing chunk 1 of 1

  |                                                                            
  |                                                                      |   0%
  |                                                                            
  |======================================================================| 100%
• Formatting the tail data...
Error in round(result$tail_length, digits = 2) : 
  non-numeric argument to mathematical function
Calls: find_tails
In addition: Warning messages:
1: `cols` is now required when using unnest().
Please use `cols = c()` 
2: In rm(polya_fastq) : object 'polya_fastq' not found
3: Unknown or uninitialised column: `tail_length`. 
Execution halted

Could you please help me identify what I might be doing wrong here?

Thank you in advance :)

[adnaniazi]

Hi,

This error essentially means that not single valid polyA tail was found in all the reads which you presented to tailfindr.

Best, Adnan

[jchang97]

Thank you so much Adnan!