boutroslab / CRISPRAnalyzeR

CRISPRAnalyzeR: interactive analysis, annotation and documentation of pooled CRISPR screens
GNU General Public License v2.0
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lentiCRISPv2 wrong regular expression #43

Open jacboero opened 6 years ago

jacboero commented 6 years ago

Hey..

Foremost thanks for the work on this. I just installed it today and try to give some feedback if I am more familiar with all functions.

What I can say so far - and it is not a big issue, but I think that at least for some of the libraries out there the regular expression for lenticrispv2 is wrong. I used the full GeckOv2 KO-library and it took me a while to figure out, why I was getting no sgRNAs assigned.

if one uses ACCG(.{20,21})G instead of the CACC(.{20,21})G it works...

jwinter6 commented 6 years ago

thanks, will be fixed in next maintanence update.