Hello,
First thank you very much for creating this tool and for letting it freely accessible online. I recently performed a CRISPR screen and used CRISPRanalyzer to analyse my sequencing data. I just wanted to let you know I detected a mistake in the settings. The default settings for gRNA extraction from the FASTQ file for lentiguide (Gecko library) is ACC{20,21}G. However these settings fail to extract the gRNA sequence. The settings should be instead ACCG{20,21}G. Luckily these are the settings that are proposed for another vector in CRISPRanalyzer (pLCKO), which I could select to extract gRNA sequences after downloading custom library.
Thanks again for providing this great tool!
Eric
Hello, First thank you very much for creating this tool and for letting it freely accessible online. I recently performed a CRISPR screen and used CRISPRanalyzer to analyse my sequencing data. I just wanted to let you know I detected a mistake in the settings. The default settings for gRNA extraction from the FASTQ file for lentiguide (Gecko library) is ACC{20,21}G. However these settings fail to extract the gRNA sequence. The settings should be instead ACCG{20,21}G. Luckily these are the settings that are proposed for another vector in CRISPRanalyzer (pLCKO), which I could select to extract gRNA sequences after downloading custom library. Thanks again for providing this great tool! Eric