Closed wclee47 closed 1 year ago
Hi @wclee47 thank you for your question. The -o
is supposed to be the output file prefix whereas -s
is the read_group name in the bam header. I think my example command line is confusing. It may be easier to understand if -o
and -s
are the same.
Hi ruolin, thanks for the explanation.
Yes, your example was a bit confusing because the output prefix is set to be "trim_outprefix" and the resulting bam file has "trim_outprefix.sample_A". Hope it to be more clarified.
Anyway thank you very much for the help!
Won-Chul
Hi, when running codec trim, it seems that "-s" parameter doesn't work. When I followed the instructions here:
codec trim -1 demux_outprefix.sample_A.1.fastq.gz -2 demux_outprefix.sample_A.2.fastq.gz -o trim_outprefix -u 3 -U 3 -f 2 -t 2 -s sample_A
It produces the trimmed BAM named "trim_outprefix.trim.bam", and doesn't include "sample_A" in the name.
Could you please check the code.
Thanks!
Won-Chul