broadinstitute / pilon

Pilon is an automated genome assembly improvement and variant detection tool
GNU General Public License v2.0
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Not detecting 100bp insertion #109

Open ybendana opened 4 years ago

ybendana commented 4 years ago

Using art_illumina, I simulated Miseq 150 bp paired-end reads having a mean fragment size of 350 bp for a modified pKLAC1 vector. Pilon is unable to detect a 100 bp insertion, although it is able to detect a 50bp insertion. Is this to be expected?