Closed lfaller closed 5 years ago
Hi Lina, I'm finally catching up on Pilon support. Thanks for writing!
Extra coverage shouldn't hurt, except in terms of compute time. Generally, I find there's not much to be gained beyond 200x or so with Illumina sequencing.
Good luck!
Hello,
I have some very deeply sequenced samples. In order to improve processing times I usually downsample them using bbnorm to about 100x coverage.
The wiki states that "... total sequence coverage should be 50x or greater, though deeper total coverage of >100x is beneficial".
Would you recommend ever downsampling? Is there an upper limit (i.e. 1000x) where extra coverage does not add anything else?
Thanks for any advice! ~Lina