gwaybio
commented
5 years ago - The confluence of cells appear to be a big issue
- The current molecular pipeline is to seed at uniform density
- If we generate additional plates, seeded at different densities, we can test which densities are best for specific clones
- I will also add cell count to the subcluster morpheus heatmap (see #33)
- Next steps:
- @bethac07 and team will run image analysis pipeline on new data
- @mekelley and Adi (we need to get Adi on github! 😄 ) will generate new data with different seeding concentrations (see above)
- I will send out list of top 10-15 features (based on certain metrics) to send to biologists for interpretation (@bethac07, @mekelley, Adi, and @annecarpenter) (see #34)