Notes from Meeting 1/8/20

[gwaybio]

Documenting notes here that I previously transcribed

• Cloning procedure was clarified in #45
• Next steps computationally
  • Look at features that differentiate resistant and wild-type
  • Are these features consistent in different batches?
  • Figure out the best way to merge datasets together
• Next steps molecularly
  • Collect cell painting data in a new cell line (this will require additional troubleshooting to onboard new cell line)
  • ~2 to 4 months for new cell line
    • Broad may help with acquisition?
  • Collect resistance mechanisms from new drug
  • ~2 months for another drug
• Potential high-level outcomes:
  • We find a clear resistance signature that helps form hypothesis
  • The resistance signature is a mystery and we'll need to figure out next steps

[AnneCarpenter]

Next time we meet we should discuss that it's probably trivial to tell the difference between sensitive vs resistant cells when they've been treated with a cancer drug (death is easy to detect). The interesting thing will be to tell the difference before they've been treated, or within a few minutes/hours of being treated. Are we collecting data for testing these?

[gwaybio]

We discussed on the call last week (more notes in #47 ) that the treatments are only 4 hours post treatment. We also discussed that we could experiment with timing and see if we can collect much sooner. This is an exciting variable to track and test!