Open lzyskyline opened 4 months ago
陈老师，您好，我在跑geta的第二步2.homolog_prediction时，出现以下报错： failed to execute: /public/home/geta/bin/homolog_prediction --tmp_dir /public/home/project/1_cooperate/FX-geta/FX.tmp/2.homolog_prediction --cpu 30 --identity 0.2 --evalue 1e-9 --homolog_coverage 0.3 --max_hits_num_per_match_region 10 --max_hit_num_per_single_species 2 --method exonerate,genewise,gth --threshod_ratio_of_intron_Supported_times 0.0 --genetic_code 1 --output_alignment_GFF3 /public/home/project/1_cooperate/FX-geta/FX.tmp/2.homolog_prediction/homolog_alignment.gff3 --output_raw_GFF3 /public/home/project/1_cooperate/FX-geta/FX.tmp/2.homolog_prediction/homolog_prediction.raw.gff3 /public/home/project/FX-geta/FX.tmp/homolog.fasta /public/home/project/FX-geta/FX.tmp/genome.fasta > /public/home/project/FX-geta/FX.tmp/2.homolog_prediction/homolog_prediction.gff3 2> /public/home/project/FX-geta/FX.tmp/2.homolog_prediction/homolog_prediction.log 随后我单独运行了genewise，出现以下报错： genewise /public/home/project/1_cooperate/FX-geta/FX.tmp/2.homolog_prediction/b.hitToGenePrediction/chunk0/Chr01.49687_50382.start20354.AT2G01050_1.homolog.fasta /public/home/project/1_cooperate/FX-geta/FX.tmp/2.homolog_prediction/b.hitToGenePrediction/chunk0/Chr01.49687_50382.start20354.AT2G01050_1.genome.fasta -codon /public/home/project/1_cooperate/FX-geta/FX.tmp/2.homolog_prediction/b.hitToGenePrediction/codon.table -trev -gff -genesf > /public/home/project/1_cooperate/FX-geta/FX.tmp/2.homolog_prediction/b.hitToGenePrediction/chunk0/genewise/Chr01.49687_50382.start20354.AT2G01050_1.gff Warning Error Unable to open gene.stat as gene stats file Warning Error Could not read gene statistics in gene.stat Warning Error Could not open BLOSUM62.bla as a filename for read Blast matrix Warning Error Could not read Comparison matrix file in BLOSUM62.bla Fatal Error Could not build objects! 请问，应该如何解决这个问题？谢谢
你好，我也遇到了一样的报错，请问你解决了吗？
dayuwww
commented
3 weeks ago dayuwww
commented
3 weeks ago
陈老师，您好，我在跑geta的第二步2.homolog_prediction时，出现以下报错： failed to execute: /public/home/geta/bin/homolog_prediction --tmp_dir /public/home/project/1_cooperate/FX-geta/FX.tmp/2.homolog_prediction --cpu 30 --identity 0.2 --evalue 1e-9 --homolog_coverage 0.3 --max_hits_num_per_match_region 10 --max_hit_num_per_single_species 2 --method exonerate,genewise,gth --threshod_ratio_of_intron_Supported_times 0.0 --genetic_code 1 --output_alignment_GFF3 /public/home/project/1_cooperate/FX-geta/FX.tmp/2.homolog_prediction/homolog_alignment.gff3 --output_raw_GFF3 /public/home/project/1_cooperate/FX-geta/FX.tmp/2.homolog_prediction/homolog_prediction.raw.gff3 /public/home/project/FX-geta/FX.tmp/homolog.fasta /public/home/project/FX-geta/FX.tmp/genome.fasta > /public/home/project/FX-geta/FX.tmp/2.homolog_prediction/homolog_prediction.gff3 2> /public/home/project/FX-geta/FX.tmp/2.homolog_prediction/homolog_prediction.log 随后我单独运行了genewise，出现以下报错： genewise /public/home/project/1_cooperate/FX-geta/FX.tmp/2.homolog_prediction/b.hitToGenePrediction/chunk0/Chr01.49687_50382.start20354.AT2G01050_1.homolog.fasta /public/home/project/1_cooperate/FX-geta/FX.tmp/2.homolog_prediction/b.hitToGenePrediction/chunk0/Chr01.49687_50382.start20354.AT2G01050_1.genome.fasta -codon /public/home/project/1_cooperate/FX-geta/FX.tmp/2.homolog_prediction/b.hitToGenePrediction/codon.table -trev -gff -genesf > /public/home/project/1_cooperate/FX-geta/FX.tmp/2.homolog_prediction/b.hitToGenePrediction/chunk0/genewise/Chr01.49687_50382.start20354.AT2G01050_1.gff Warning Error Unable to open gene.stat as gene stats file Warning Error Could not read gene statistics in gene.stat Warning Error Could not open BLOSUM62.bla as a filename for read Blast matrix Warning Error Could not read Comparison matrix file in BLOSUM62.bla Fatal Error Could not build objects! 请问，应该如何解决这个问题？谢谢