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cjfields / Bio-Tools-Primer3Redux

A reimplementation of BioPerl's Primer3-related code for primer3 v1 and v2
http://bioperl.org
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Run w/o a sequence to check existing primers #9

Closed fschwach closed 13 years ago

fschwach commented 13 years ago

primer3 task check_primers can be run w/o giving a SEQUENCE_TEMPLATE to check existing primers for dimer formation etc. I have made changes to enable this behaviour in Primer3Redux. When running w/o a sequence, the primer task must be check_primers. A mock sequence of Ns and default length is created and the given primer sequences are substituted into this sequence at the beginning, middle and end so that a Bio::Seq object can be retrieved that gives the correct primer sequences and distances.

fschwach commented 13 years ago

I just added the thermodynamic parameters as well.