Example of using omit_AA?

[marissadolorfino]

I am trying to make single point mutations in a sequence, where I will mutate the single position to all 20 canonical amino acids. I am trying to use design_only_positions and omit_AA, where the first list will include only one position and omit_AA will include all AA except for one.

I am getting an error regarding omit_AA. Is there an example of how to use omit_AA correctly? Thank you!

[dauparas]

Try using the --omit_AAs flag; e.g. to exclude everything except alanine use --omit_AAs "CDEFGHIKLMNPQRSTVWYX".

[marissadolorfino]

Oh I see, thank you!