Open evaleiyuhang opened 9 months ago
16/11/2023 19:37
icc day1 11:30-15:00 🫐
Immunostaining protocol
ICC Day2 08:00-14:00 🫐🫐🫐🫐
"PFA: 24✖️24w,clean with \4% PFA\ 500ul,friege 【calculate】: \4%PFA\:24/24w✖️500ul=12000ul, 、\37%PFA\ to 12000ul \4%PFA\:12000ul/37/4=1297ul, 、\PBS\:12000-1297ul \37% PFA\=10721ul 、10721ul \PBS+1297ul \37%PFA\=12000UL \4%PFA\ 【Protocol】
Sample preparation. (immunocytochemistry)
icc day0 Sample preparation. (immunocytochemistry)
icc day1 21/11/23 11:30-15:00 🫐 Immunostaining protocol
ICC Day2 22/11/23 11:00-15:00 🫐🫐🫐🫐
imagecupture 23/11/23 14:30-15:30
channel:488,555 exposure:auto , exposure cvi,tiff save in usb
imagecupture 27/11/23 17:30-18:30
channel:488,555,dapi time:488 time 200us, 555 time 100 us, dapi 75us, exposure cvi,large tiff(full + single channel) save in onedrive
icc day1 11:30-15:00 🫐
Immunostaining protocol
ICC Day2 08:00-14:00 🫐🫐🫐🫐