fibertools-rs
fibertools-rs a CLI tool for creating and interacting with Fiber-seq BAM files. For more details read the book.
Install
fibertools-rs is avalible through bioconda and can be installed with the following command:
conda install bioconda::fibertools-rsHowever, due to size constraints in bioconda this version does not support contain the pytorch libraries or GPU acceleration for m6A predictions. m6A predictions will still work in the bioconda version but may be much slower. If you would like to use m6A prediction and GPU acceleration, you will need to install using the directions in the fibertools book.
Usage
ft --helpHighlighted subcommands for fibertools-rs
ft predict-m6a
Predict m6A positions using HiFi kinetics data and encode the results in the MM and ML bam tags. Docs for predict-m6a.
ft add-nucleosomes
Add nucleosomes to a bam that file already contains m6a predictions. Note, this process is also run in the background during predict-m6a, so it is unnecessary to run independently unless you want to try new parameters for nucleosome calling. Docs for add-nucleosomes.
ft extract
Extracts Fiber-seq data from a bam file into plain text. Docs for extract.
ft center
Center Fiber-seq reads (bam) around reference position(s). Docs for center.
ft footprint
Footprint Fiber-seq reads (bam) around reference motifs(s). Docs for footprint.
Python API (pyft)
The python API is still in development and not stable; however, you can find the current code progress in the py-ft folder. More information available at readthedocs.
Cite
Jha, A., Bohaczuk, S. C., Mao, Y., Ranchalis, J., Mallory, B. J., Min, A. T., Hamm, M. O., Swanson, E., Dubocanin, D., Finkbeiner, C., Li, T., Whittington, D., Noble, W. S., Stergachis, A. B., & Vollger, M. R. (2024). DNA-m6A calling and integrated long-read epigenetic and genetic analysis with fibertools. Genome Research. https://doi.org/10.1101/gr.279095.124
Contributing
If you would like to contribute to fibertools-rs, please see the CONTRIBUTING.md file for more information.