NTR: peroxynitrite reductase

geneontology / go-ontology

Source ontology files for the Gene Ontology

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NTR: peroxynitrite reductase #8278

Closed gocentral closed 9 years ago

gocentral commented 13 years ago

Hello, could you please add:

function "peroxynitrite reductase activity" DEF Catalysis of the reduction of peroxynitrite to nitrite, with possible creation of oxygen or water, using NADPH as reduction equivalent IS_A GO:0016708 oxidoreductase activity, acting on paired donors, with incorporation... REF PMID 11001062

Regards, ralf

Reported by: rwst

Original Ticket: geneontology/ontology-requests/8065

gocentral commented 13 years ago

assigned_to: nobody --> gomidori

Original comment by: mah11

gocentral commented 13 years ago

I forgot to add the synonym peroxynitritase. Also, the enzyme is a peroxiredoxin. It doesn't use NADPH itself---it's the accompanying disulfide reductase that uses it. So, to separate both activities, this reaction can be written

2 R-SH + ONOO- = R-SS-R + NO2- + H2O

Original comment by: rwst

gocentral commented 13 years ago

Hello,

I have added

peroxynitrite reductase activity GO:0072541

with the parent peroxiredoxin activity GO:0051920.

If the peroxynitrite reductase reaction doesn't directly use NADPH, is it still correct to use GO:0016708 as a parent? If not, is there another term use instead (in addition to GO:0051920)?

thanks, midori

Original comment by: mah11

gocentral commented 13 years ago

status: open --> open-accepted

Original comment by: mah11

gocentral commented 13 years ago

No, I can't think of another parent. It's fine as you made it.

Thanks for you work, ralf

Original comment by: rwst

gocentral commented 13 years ago

OK, in that case I can close this item. Thanks!

Midori

Original comment by: mah11

gocentral commented 13 years ago

status: open-accepted --> closed-accepted

Original comment by: mah11

gocentral commented 13 years ago

Hi Ralf,

Whereabouts in the paper does the reaction comes from? I'd like to cross-reference it with other databases.

Original comment by: girlwithglasses

gocentral commented 13 years ago

Not sure what you mean. This activity is certainly missing in EC or Metacyc databases.

ralf

Original comment by: rwst

gocentral commented 13 years ago

Can you cut and paste the bit of text from whence you got the reaction equation?

Original comment by: girlwithglasses

gocentral commented 13 years ago

The PMID I gave in the original request does not present a ready-to-use equation. However, please read the snippets:

"Each molecule of peroxynitrite led to the oxidation of 2 Cys (Fig. 2a inset)."
" Lower concentrations of peroxynitrite only caused dis- ulphide bond formation (Fig. 2b). Thus, the first residues in AhpC to react with peroxynitrite are Cys, not Tyr, and the reaction leads to reversible disulphide bonding."
"Spontaneous decomposition of peroxynitrite in pure solution leads to the accumulation of nitrate (D70%) and nitrite (D30%)1. In contrast, wild-type AhpC and C165A caused the nearly quanti- tative conversion of peroxynitrite into nitrite (...)"
They also ruled out nitrosation in favor of oxidation. They found one oxygen atom incorporated in the intermediate.
From this all follows naturally the equation I gave in a comment.

ralf

Original comment by: rwst