liangkaiye
commented
6 years ago Pindel is a variant discovery tool so that it is stringent in read selection to make a call. At the same time, it does counting ref supporting allele aggressively. A separate allele counting script could correct this.
Hi，
I am using Pindel to call indels in our samples, Our samples are tumor-normal samples, and it's target sequencing data, the library was constructed by capturing.
I used BWA mem to map the trim data with hg19 reference by default parameter. After getting the sorted bam file, I used samtools to remove duplication in the data. But samtools don't work well with duplication, It may not well removed.
And then using Pindel to call indels. Cause we have the answers of the sample, the VAF in the pindel vcf are much lower than the answers, we use the cut-off value of 1% for somatic variations, therefore many indels are filtered out by this threshold.
I just adjust the -w to 2 and turn off the SV calling. Other parameters are all by default. My questions are why this situation would happen? and how to correct the parameter to get the right VAF of indels.
The following four results are from Pindel, The VAF of this four indels are 0.08, 0.15, 0.03, 0.12. But according to our results, the VAF are 0.002, 0.009, 0.0013 and 0.0032 respectively.
Thank you for your time.
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jinxinhao0627
Hi，
I am using Pindel to call indels in our samples, Our samples are tumor-normal samples, and it's target sequencing data, the library was constructed by capturing.
I used BWA mem to map the trim data with hg19 reference by default parameter. After getting the sorted bam file, I used samtools to remove duplication in the data. But samtools don't work well with duplication, It may not well removed.
And then using Pindel to call indels. Cause we have the answers of the sample, the VAF in the pindel vcf are much lower than the answers, we use the cut-off value of 1% for somatic variations, therefore many indels are filtered out by this threshold.
I just adjust the -w to 2 and turn off the SV calling. Other parameters are all by default. My questions are why this situation would happen? and how to correct the parameter to get the right VAF of indels.
The following four results are from Pindel, The VAF of this four indels are 0.08, 0.15, 0.03, 0.12. But according to our results, the VAF are 0.002, 0.009, 0.0013 and 0.0032 respectively.
Thank you for your time.