I want to use DSS to merge four samples with the same CpGs loci by makeBSseqData function.
Two groups, each group has two replicates, "C1","C2", "N1", "N2".
When merging them together, it works but error information reminds me that
1: In makeBSseqData(list(data_total3[[1]], data_total3[[3]], ... :
CG positions in chromosome chr1 is not ordered. Reorder CG sites.
I tried to sort them before this step but still doesn't work.
And I referred to here. Here you said The warning message appears when the input data are not sorted by CG site locations. It won't affect your data analysis.
Hello there,
I want to use DSS to merge four samples with the same CpGs loci by makeBSseqData function.
Two groups, each group has two replicates, "C1","C2", "N1", "N2".
When merging them together, it works but error information reminds me that
1: In makeBSseqData(list(data_total3[[1]], data_total3[[3]], ... :
CG positions in chromosome chr1 is not ordered. Reorder CG sites.
I tried to sort them before this step but still doesn't work.
And I referred to here. Here you said The warning message appears when the input data are not sorted by CG site locations. It won't affect your data analysis.
So I want to know if I can ignore this message ?
Thanks.