hartleys / QoRTs

Quality of RNA-Seq Toolset
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understanding Mapping location rates #58

Closed ccshao closed 6 years ago

ccshao commented 6 years ago

I have a naive question on the " Mapping location rates" plot. Basically, how should I understand and add the rates of each categories? For example, in the plot: https://hartleys.github.io/QoRTs/archive/v1.0.22/QoRTs-vignette/node9.html#subsec:plotDesc-14d If simplely sum the rate of each categories, then the total rate will be larger than 1. My questions are:-

hartleys commented 6 years ago

UniqueGene + AmbigGene + NoGene =1.

All the others are subsets of one of those 3, as indicated by their name.

So, for example, "no gene, intronic" is a subset of "no gene", since we only count a read as belonging to a gene if it appears on an exon.

On Mar 2, 2018 11:29 AM, "shao" notifications@github.com wrote:

I have a naive question on the " Mapping location rates" plot. Basically, how should I understand and add the rates of each categories? For example, in the plot: https://hartleys.github.io/QoRTs/archive/v1.0.22/QoRTs-vigne tte/node9.html#subsec:plotDesc-14d If simplely sum the rate of each categories, then the total rate will be larger than 1. My questions are:-

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ccshao commented 6 years ago

Great, the plot makes a lot of sense and very helpful!