plot multiple bigwig data in one heatmap?

[junjunlab]

Hello, thank you for your such a great package. I have multiple bigwig files, I want to plot multiple bigwig files in one heatmap, but the normalizeToMatrix function can only accept one signal file. So how should I do to deal with these data? Can you help me ? I am looking forward to your reply!

[jokergoo]

What do you mean by "plot multiple bigwig files in one heatmap"? Can you imagine how the plot will look like?

[kaanokay]

Hi @jokergoo

Thank you for developing such a good package.

I think, @junjunlab meant that when we have multiple signal data (coming from multiple different samples) how to draw a heatmap where we can represent all samples with different lines (each line is differently colored for each sample) on top of annotation for the same target region.

That is, instead of having multiple heatmaps for all signal data, how to have a heatmap showing methylation trend for all samples as lines on top of heatmap by targeting same target region for all samples.

A heatmap like below:

Is that possible with your package?

Because for each signal data we have to create normalized matrix with target regions and combine several heatmaps by adding "+" character. How to pass this and get one heatmap for all samples?

Best.