Dear developers, we would like to do cross-feeding simulations for bacteria growing in Mega Medium. I am unsure how to convert this information into a format that can be used with gapseq. Could you clarify how I can determine the flux rates and cpd-ids?
Thank you!
# Component Amount (in 500 ml)
Milli-Q water (dH2O) 410 ml
1 M Potassium phophate buffer, pH 7.2 50 ml
TYG salts solution 20 ml
Tween 80 1 ml of 25% (v/v)
SCFA supplement 1.4 ml
0.8% (w/v) CaCl2 500 ul
FeSO4 7H20 500 ul of 0.4 mg/ml
Resazurin 2 ml of 0.25 mg/ml
Trypticase Peptone (BBL) 5 g
Yeast Extract (Bacto) 2.5 g
Meat Extract 2.5 g
L-Cysteine hydrochloride 0.25 g
D-(+)-Glucose 1 g
D-(+)-Cellobiose 0.5 g
D-(+)-Maltose monohydrate 0.5 g
D-(-)-Fructose 0.5 g
Soluble Starch 0.25 g
Vitamin K + Hematin (1%) 5 ml
Trace mineral supplement 5 ml
Vitamin supplement 5 ml
Dear developers, we would like to do cross-feeding simulations for bacteria growing in Mega Medium. I am unsure how to convert this information into a format that can be used with gapseq. Could you clarify how I can determine the flux rates and cpd-ids? Thank you!