Nanopolish polyA on RNA004 data

jts / nanopolish

Signal-level algorithms for MinION data

MIT License

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Nanopolish polyA on RNA004 data #1136

Closed rugilemat closed 8 months ago

rugilemat commented 8 months ago

Hi,

I want to estimate the polyA length in my direct RNA samples but they're run with RNA004 kit and new RNA flow cells. I've used f5c for indexing and event aligning for my other applications, so I'm just wondering if nanopolish polyA would be compatible with this?

jts commented 8 months ago

Hi,

Sorry but RNA004 is not supported by the poly-A tail caller since dorado now estimates poly-A tail lengths.

Jared

rugilemat commented 8 months ago

I see, thanks for such a quick response!